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KMID : 1161420150180121307
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Journal of Medicinal Food
2015 Volume.18 No. 12 p.1307 ~ p.1316
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Quercetin Induces Dose-Dependent Differential Morphological and Proliferative Changes in Rat Uteri in the Presence and in the Absence of Estrogen
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Shahzad Huma
Giribabu Nelli
Sekaran Muniandy
Salleh Naguib
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Abstract
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Quercetin could have profound effects on uterine morphology and proliferation, which are known to be influenced by estrogen. This study investigated the effect of quercetin on these uterine parameters in the presence and in the absence of estrogen. Ovariectomized adult female rats received peanut oil, quercetin (10, 50, and 100?mg/kg/day), estrogen, or estrogen+quercetin (10, 50, or 100?mg/kg/day) treatment for 7 consecutive days. At the end of the treatment, uteri were harvested for histological and molecular biological analyses. Distribution of proliferative cell nuclear antigen (PCNA) protein in the uterus was observed by immunohistochemistry. Levels of expression of PCNA protein and mRNA in uterine tissue homogenates were determined by Western blotting and real-time polymerase chain reaction, respectively. Our findings indicated that administration of 10?mg/kg/day of quercetin either alone or with estrogen resulted in decreased uterine expression of PCNA protein and mRNA with the percentage of PCNA-positive cells in uterine luminal and glandular epithelia markedly reduced compared with estrogen-only treatment. Changes in uterine morphology were the opposite of changes observed following estrogen treatment. Treatment with 100?mg/kg/day of quercetin either alone or with estrogen resulted in elevated PCNA protein and mRNA expression. In addition, the percentages of PCNA-positive cells in the epithelia, which line the lumen and glands, were increased with morphological features mimicking changes that occur following estrogen treatment. Following 50?mg/kg/day quercetin treatment, the changes observed were in between those changes that occur following 10 and 100?mg/kg/day quercetin treatment. In conclusion, changes in uterine morphology and proliferation following 10?mg/kg/day quercetin treatment could be attributed to quercetin's antiestrogenic properties, while changes that occur following 100?mg/kg/day quercetin treatment could be attributed to quercetin's estrogenic properties.
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KEYWORD
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quercetin, uterine morphology and proliferation
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